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Clinical Significance : Total testosterone circulates primarily as protein-bound (approximately 60% bound to sex hormone binding globulin (SHBG) and 50% to albumin). Only 2-3% exists in free, biological active form. Testosterone is weakly bound to albumin and can be reversed easily, therefore albumin bound and free testosterone are considered to be bioavailable testosterone. The highest testosterone level peaks at 30 to 40 years of age in adult men. The levels start to decline steadily after the fourth or fifth decade of adult male life. Decreased levels of testosterone and/or free testosterone indicate partial or complete hypogonadism. It is important to determine if low levels of testosterone are due to aging or a pathological disorder. In adult men, testicular or androgen abuse might be suspected if testosterone levels exceed the upper limit of the normal range by more than 50%.