Zhuzhou Yuancheng Hezhong Technology Development Co., Ltd.
Zhuzhou Yuancheng Hezhong Technology Development Co., Ltd. was
founded in 2013, a branch of "Yuancheng" Group. Which is located in
Tianyuan District, Zhuzhou City-The Economic Centre of Changsha
Zhuzhou and Xiangtan Cities Group. It is a large high-t tech
chemical enterprise which specialized in fine chemicals , flavors
and fragrances, food additives, pharmaceutical raw material and
intermediates. Companies affiliated factory has advanced production
equipment and plants, perfect quality assurance and control system,
whole set of High Pressure Liquid Chromatography/Gas
Chromatography/UV Spectrophotometer to conduct effective product
analysis and monitoring. Company factory has passed ISO9001 quality
system certification and international KOSHER certification.
Zhuzhou Yuancheng Hezhong Technology Development Co., Ltd. based in chemical industry, lead by food chemical market. Complete e-commerce networks make guarantee for timely service to the world customers. To meet the needs of customers, each product is elite, which is recommended and selected by professional and experienced technicals for customers. The company has independent import and export rights, products exported to foreign markets with high quality and good service, established good business relationship with other countries in Europe, America, Asia, etc. All the staffs will make unremitting efforts to strive for outstanding quality, make excellent brand and service the market by first-class products.
D-Aspartic acid (D-Asp) and nitric oxide (NO) are two biologically active molecules playing important functions as neurotransmitters and neuromodulators of nerve impulse and as regulators of hormone production by endocrine organs. We studied the occurrence of D-Asp and NO as well as their effects on testosterone synthesis in the testis of boar. This model was chosen for our investigations because it contains more Leydig cells than other mammals. Indirect immunofluorescence applied to cryostat sections was used to evaluate the co-localization of D-Asp and of the enzyme nitric oxide synthase (NOS) in the same Leydig cells. D-Asp and NOS often co-existed in the same Leydig cells and were found, separately, in many other testicular cytotypes. D-Asp level was dosed by an enzymatic method performed on boar testis extracts and was 40+/- nmol/g of fresh tissue. NO measurement was carried out using a biochemical method by NOS activity determination and expressed as quantity of nitrites produced: it was +/- nmol/mg of tissue. The effects of the two molecules on steroid hormone production were evaluated by incubating testis homogenates, respectively with or without D-Asp and/or the NO-donor L-arginine (L-Arg). After incubation, the testosterone presence was measured by immunoenzymatic assay (EIA). These in vitro experiments showed that the addition of D-Asp to incubated testicular homogenates significantly increased testosterone concentration, whereas the addition of L-Arg decreased the hormone production. Moreover, the inclusion of L-Arg to an incubation medium of testicular homogenates with added D-Asp, completely inhibited the stimulating effects of this enantiomer. Our results suggest an autocrine action of both D-Asp and NO on the steroidogenetic activity of the Leydig cell.